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The secondary structures were predicted by using default parameters.
This refined protein structure was used for receptor grid generation by using default parameters.
Annotation of all sequences was performed by using default parameters on the two ranges of length previously described.
By using default parameters, we did not fine-tune all possible options available in the individual software applications.
The PCR product Sanger sequences were aligned to the reference in SeqMan Pro software (DNAStar) for validation by using default parameters.
SNPs and indels were used for further analysis if they passed the Cortex population filter/site classifier by using default parameters.
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Homology modelling of PschSOD was prepared by using default parameter at SWISS model workspace (http://swissmodel.expasy.org/) [ 53, 54].
In addition, CLUSTAL-W alignments were converted to PHYLIP format and trees computed by the maximum likelihood method implemented by PROML using default parameters [ 90].
CpG island were detected by CpGPlot using default parameters (http://www.ebi.ac.uk/Tools/seqstats/emboss_cpgplot/).ac.uk/Tools/seqstats/emboss_cpgplot/
Blast results were obtained by blastp using default parameters.
Sequences were aligned by ClustalW using default parameters [ 42] resulting in an alignment of 3455 nt.
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by using deformation parameters
by using default passwords
by using find parameters
by using reservoir parameters
by using lamination parameters
by using default rules
by selecting default parameters
by using default options
by using design parameters
by applying default parameters
by modifying default parameters
by using facade parameters
by using person parameters
by using default settings
by using transport parameters
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