Cellular and molecular events were easily visualised using conventional microscopy.
This is simply not possible by using conventional fluorescence microscopy where the resolution of the light microscope is limited to ~ 250 nm laterally and ~ 500 nm axially.
The present study was undertaken to investigate the relation between IPC and Bcl-2 expression immunohistochemically and apoptosis (by using conventional light microscopy, immunohistochemical staining for cytokeratin 18 [M30 cytodeath Ab], DNA agarose gel electrophoresis) in the intestine.
This result supports a study by Krippendorf and Lough [ 20], which demonstrated a significant improvement in the identification of tissue structure and case analysis exam performance by students who learned using virtual microscopy when compared with those who used conventional microscopy.
In this paper, PbTiO3 thin films with variant thicknesses are deposited on (110 -oriented GdScO3 substrates by Pulsed Laser Deposition (PLD) and investigated by using conventional transmission electron microscopy (TEM) and Cs-corrected Scanning TEM.
Although this problem was partially resolved by using conventional histology and electron microscopy [12], [13], a more convincing examination, such as immunohistochemistry with a reliable molecular marker of lymphatic endothelial cells, LYVE-1 [15], [16], [16], had not been tried until this research.
This precipitation and the typical defect structures generated within the DO19 compound were investigated by using conventional and high-resolution transmission electron microscopy (CTEM and HRTEM).
Isolates were identified by using conventional methods.
Mixing of SCC cells with cancer-associated fibroblasts can allow the study of their interaction and reciprocal influence, which can be followed in real time by intradermal ear injection using conventional fluorescent microscopy.
The phenotype and precise location of these cells, as judged by LTA staining, was difficult to assess using conventional fluorescence microscopy.
