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Then, a new method is proposed for minimizing cell-load variation in the mentioned condition by using control lines.
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This was resolved by using control line estimates of the resident seed bank, estimates of the proportion of sown seed that was truly hybrid and measures of B. rapa vital rates from the relevant experimental lines in a series of equations to calculate true hybrid vital rates using least squares estimation (Supplementary Data Methods S2).
By using positive control cell lines with known truncating mutations, we have determined the optimal concentration of caffeine that results in significant stabilisation of target genes, which is suggestive of successful inhibition of the nonsense-mediated mRNA decay pathway.
We have taken this into account in our experiments by using a control cell line (pLKO vector cells) that was cultured in the same puromycin-containing media as our shRNA cells.
In addition, the filing documents the family's purchase of Adelphia stock through entities it controlled by using credit lines guaranteed by the company.
However, there are two limitations by using droop control when considering line resistance.
This paper used Pro/E's datum-graph function, to create the follower's displacement line graph, and to control cam profile by using the displacement line graph, the line translating tip follower cam mechanism was wholly parametric designed.
Outline the ears by using curve lines.
The currently used control algorithms face difficulties in suppressing abnormal spectral lines caused by this inverse problem.
Thinking about software made us think about interface design, and this interface design was tested by using software such as controlling lines for clock signals to the CCD sensor, the AD9822, and control lines for buffer chips.
The w 1118 line was used as control line.
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