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The immunofluorescence images were digitally recorded by using confocal microscope.
DAPI was used for nuclear staining and then analyzed by using confocal microscope.
For each condition, 20 single-cell images were collected by using confocal microscope Zeiss LSM 510 (Zeiss, Göttingen, Germany) and processed for colocalization analysis.
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Images based on the stack of consecutive 2 µm sections (usually 15 sections) were generated by using confocal microscopy (Leica SP5 AOBS Confocal Microscope).
The fixed cells were washed with PBT, blocked with 3% BSA followed by antibody incubation and analysis using confocal microscope.
The time course of fluorescence changes in wild-type Jacob-GFP expressing CA1 neurons was acquired by scanning of 10 planes using confocal microscope every 2 min. The distance between the first and the last optical plane was about 70 µm.
We also assessed the effect of AuNPs on the mitochondrial morphology by using confocal fluorescence microscope, and found that in contrast to the normal long tubular mitochondria in control cells, the mitochondria of the cells treated with 13 nm AuNPs became punctate and swollen (Fig. 4e), further demonstrating the notion that 13 nm AuNPs induced damage of mitochondria.
After conditioning of the honeycomb film and the flat film in DMEM containing 10% foetal bovine serum (FBS) for 72 h at 37 °C in 5% CO2 atmosphere, the adsorbed fibronectin-FITC and albumin-Texasred on the honeycomb films was observed by using confocal laser scanning microscope (CLSM).
The percentage of BrdU-labeled cells that expressed NeuN was determined throughout the DG by using a confocal microscope with HeNe and Argon lasers (Nikon PCM 2000).
[34] To test whether Meltrin β regulates the internalization of the ephrin-A5-EphA4 complex, we added ephrin-A5-Fc or human Fc fragment (as a negative control) to EphA4-HA transformants, and analyzed the complex with anti-Fc fragment antibodies by using a confocal microscope (Fig. 6).
Images were obtained at 0, 6, 12 and 24 h by using a confocal microscope.
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