Exact(6)
All these markers were detected by using commercial kit (Nanjing Jiancheng Bioeng. Inst., China), and the measurements were conducted according to the manufacture's protocols.
β2M analysis was performed by using commercial kit (DiaSorin, Saluggia, Italy) according to the manufacturer's instructions.
*EBV antibody was measured by using commercial kit Euroimmun (Medizinische Labordiagnostika, Lübeck, Germany).
Spoligotyping analysis was performed by using commercial kit (Ocimun Biosolutions) as described by Kamerbeek et al. [ 13].
Serum FFAs were determined by using commercial kit (Wako Chemicals, Neuss, Germany) on an Olympus AU640 analyzer (Olympus Diagnostika, Hamburg, Germany).
On the 30th day, blood was collected in the EDTA coated tubes for measuring glycosylated hemoglobin (HbA1C) by using commercial kit (coral HbA1C estimation kit) and blood was collected in tubes without anticoagulant for serum preparations.
Similar(54)
Other parameters were determined by using commercial kits.
To determine NF-κB, MIF, TNF-α, IL-6, IL-1β, CRP, IL-4, and IL-10 levels of the plasma, enzyme linked immunosorbent assay (ELISA) was performed by using commercial kits that are selective for mouse NF-κB, MIF, TNF-α, IL-6, IL-1β, CRP, ILIL-4, and IL-10 (Biological Marker Laboratory, Inc., USA).
Metabolites were analyzed by using commercial kits for triglycerides (Randox TR210), cholesterol (Randox CH201), urea (Randox UR457), uric acid (Randox UA233), L-lactate (Randox, LC2389), glucose (Randox GL2623), total protein (Randox TP245) and albumin (Randox, AB388).
Enzyme-linked immunoassay (ELISA) was performed to detect RANKL, OPG and C-terminal telopeptides of type I collagen in the blood serum by using commercial kits for mTRANS/RANKL/TNFSF11 Quantikine ELISA, mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kits (R&D Systems) and for RatLaps® ELISA kit (Nordic Bioscience Diagnostics A/S), respectively, according to their manufactures' instructions.
Serum CK and LDH activities were measured by using commercial kits (Shanghai Changzheng Biotech Ltd ,China).
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