Sentence examples for by using column chromatography from inspiring English sources
In the second step, C60 was purified from the mixture of fullerenes by using column chromatography.
The combined organic extract was dried over anhydrous Na2SO4, and evaporated under reduced pressures to obtain a brown gum (2.62 g), which was purified by using column chromatography.
The BU was further purified by using column chromatography packed with silica gel 60 (0.200 – 0.200 nm) as the stationary phase.
The cel7A was purified from a commercial T. reesei cellulase (Spezyme CP) by using column chromatography (dual anion exchange followed by affinity on cellulose and size-exclusion chromatography[ 21]) and loaded at 15 mg of protein per gram of biomass.
From the results it was observed that GTG, a model ghrita formulation taken for this study, can be fractionated into polar and nonpolar fractions by using column chromatography on silica gel eluted with respective polar and non-polar solvents.
The commercial cellulase preparation Celluclast® 1.5L (Novozymes, Bagsværd, Denmark) was applied to purify the cellulases CBH I, CBH II, EG I and EG II by using column chromatography, with an Äkta FPLC (GE Healthcare, Buckinghamshire, UK) which automatically measures conductivity and ultraviolet absorbance at 280 nm.
The arsenic metabolites were extracted with a chloroform-methanol solution and isolated by using column and thin-layer chromatography.
The 50 kDa CaMKK substrate was partially purified by using serial column chromatography, and was identified as Syndapin I by LC-MS/MS analysis.
The transformed metabolites were isolated from this gummy crude by using repeated column chromatography (silica gel) with petroleum ether/EtOAc gradient, affording compounds 5 (6:4 P.E./EtOAc) and 6 (1:1 P.E./EtOAc).
The transformed metabolites were isolated from this crude by using repeated column chromatography (silica gel) with petroleum ether/EtOAc gradient, afforded metabolites 2 (26 mg, petroleum ether: EtOAc, 9.5 0.5, 5.2% yield) and 3 (18 mg, petroleum ether: EtOAc, 9.8 0.2, 3.6% yield).
The detection of endogenous PfI2 in total proteins extracted from asynchronous cultures of P. falciparum were also carried out by using PfPP1 chromatography column.
