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A new approach was devised for measuring the conductivity of dispersions in the unit by using cell walls and impeller shaft as electrodes.
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Test for a cell wall stress by using cell wall stressors such as Sodium Dodecyl Sulfate (SDS, 70 μg/ml), CalcoFluor White (CFW, 50 μg/ml) and Congo Red (CR, 250 μg/ml) in Petri dishes.
In contrast, the later necessitates adding glycine to the medium to induce spheroplast formation [5], or by using cell wall-weakening agents such as isonicotinic acid hydrazide [1], [2]; moreover, laborious pretreatment steps involving chilling during cell collection and repetitious washing under anaerobic conditions are required.
Accession numbers of genes encoding proteins identified for the first time by using cell wall proteomics are underlined.
Microbial pathogens enhance infection by degrading plant cell walls using cell-wall-degrading enzymes.
The pyrithiamine resistance gene (ptrA) was selected as a dominant marker and protoplasts were prepared with high concentration (up to 108 g−1 wet weight mycelium) by using mixed cell wall-lysing enzymes.
These cells detect changes in pressure by using the wall stretch as a direct surrogate.
Health and armor energy can be replenished by picking up medical supplies and energy cells respectively, or by using wall-mounted charging devices.
by using hot wall epitaxy (HWE).
BMSCs were isolated by using the wall-sticking method.
Hence, we used enzymatic cell wall disruption for gDNA isolation.
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