Sentence examples for by using cell surface from inspiring English sources
Antibodies recognizing native M2 protein were determined by using cell surface ELISA.
In breast cancer, prospective TICs have been isolated by flow cytometry by using cell surface antigens, such as CD44 and CD24 [ 5].
After validation of the reporter system, we set out to characterize the cellular identity of PI-MECs by FACS analysis by using cell surface markers for the various epithelial populations of the mammary gland.
We first investigated the antigenic signatures of OTBCs and their parental lines by flow cytometry by using cell surface marker panels used to identify prospective breast stem and progenitor cells.
The peripheral cell subsets identified in this study were defined by using cell surface markers on the basis of peripheral cell subsets described in a previous report (Additional file 1: Table S1) [ 19].
The CSC from developmentally diverse tumors and established tumor cell lines have been isolated by using cell-surface markers expressed specifically on the CSC and are commonly defined as being highly tumorigenic on injection into immunodeficient mice.
CSCs can be isolated based on their growth properties or by sorting using cell surface antigens, metabolic markers such as CD44, CD24 and CD133, and activity of aldehyde dehydrogenase 1 (ALDH1) [49].
Shackleton and colleagues provide more definitive evidence for the existence of such a stem cell by prospectively isolating these cells using cell surface markers and performing transplantation into the cleared mammary fat pads of syngenic mice.
Isolation of specific cell types, including pluripotent stem cell (PSC -derived PSC -derived is frequently accompopulationsng cell surface antisens expressed by the cells ofrequently.
Stem cell identification strategies rely on the prospective isolation of candidate cell populations using cell surface markers, followed by in vivo functional assays in mouse models [ 5- 7].
An additional application of the HaloTag technology is cell sorting by labeling cells using cell-surface HaloTag proteins alone or in combination with cell viability markers.
