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An important simplification of our modeling approach is the implicit treatment of the spatial structure of the SAM by using cell pools that are connected via differentiation rates.
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In this study, we have improved an ICS protocol to directly ex vivo detect low to high frequencies of antigen-specific CD4+ and CD8+ T-cell responses, by using pools of long peptides.
However, no isolate was obtained from these pools by using AP61 cells.
The intensity of the induced cellular responses was measured by using pooled Env peptides; T-cell epitopes were identified by using matrix peptide pools and individual peptides.
Expression of the E1 proteins was analyzed by Western Blot using protein extracts of different cell pools.
Transduced cells were selected by using 100 μg/ml hygromycin, and resistant cells were pooled for further assays.
Cells were transfected in Optimem (Gibco) containing 100 nmol/L iRNA pools by using Dharmafect 3 (Dharmacon) for SK-BR-3 cells or Dharmafect 4 for all other cell lines for 24 hours, according to the manufacturer's protocol.
Viruses isolated from hamster heart tissue suspensions and mosquito pools were characterized antigenically by using immunofluorescence of infected cells and a panel of monoclonal antibodies described previously (20 ).
In order to test whether reducing expression of any of these miR-200c responsive genes results in rounded cell morphology, the genes were knocked down (Fig. S4) by RNA interference using oligonucleotide pools and cells were plated on top of a thick layer of collagen for imaging.
Puromycin-resistant cell pools were used in this study.
The puromycin-resistant cell pools were used for experiments.
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