For the cell proliferation assay, the cells were cultured on the samples for 1, 3, and 7 days and the cell numbers were assessed by using Cell Counting Kit-8 (CCK-8; Beyotime, Shanghai, China) assay.
Samples were normalized by using equivalent cell numbers.
Cellular analysis and manipulation of tracheal cell number using cell-cycle mutations demonstrated that tube size is not dictated by the specific number or shape of the tracheal cells that constitute it.
Our research addressed the issue of overlapping cells by using increasing numbers of cells seeded into Boyden chambers (from 10,000 to 100,000 cells).
By using differential equations, cell numbers at each division stage within different T cell compartments (from DN thymocytes to naïve T cells) in the spleen were monitored at different time points during and after ganciclovir treatment.
However, by altering these workflows and using titrated cell numbers we have succeeded in establishing an optimal RNA extraction process which yields approximately 2.5 times higher RNA yields when utilising below 3000 cells.
For xenograft assay, single-cell suspension was obtained by enzymatic digestion of the spheroids using different cell numbers in a volume of 100 μl of a 50% GFR Matrigel (BD Biosciences, Franklin Lakes, NJ, USA).
It can be difficult for servicers to find a borrower except by using a cell phone number," department officials wrote, in a report issued to the White House earlier this month.
Improvements in large-scale T-cell depletion techniques of haplo-identical peripheral mobilized stem cells (PBSC) have overcome the human leukocyte antigen (HLA) barrier by using megadose numbers of stem cells obtained by either highly purified CD34+ selection or negative depletion of T cells.
Rosiglitazone-treated human umbilical vein endothelial cells were analyzed for growth rate by use of cell number counting, 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay as well as 3H-thymidine incorporation.
