The affinity profile was determined by using cell lines transfected with sst1 5.
By using cell lines deficient of individual components of the Jak/STAT pathway or by using Jak inhibitors it was demonstrated that initial IL-24-induced apoptosis of cancer cells is independent of Jak/STAT signaling pathways and thus independent of receptor engagement [10], [45].
Studies typically investigate gene expression by using cell lines [15] [17], isolated early-stage limb mesenchyme [18], whole growth plates [19], or laser-capture microdissection, which requires fixation and decalcification protocols, and sectioning to evaluate gene expression in specific regions of the growth plate [20] [23].
These findings indicate that (i) Periostin overexpression may be frequently observed in various types of cancer, and (ii) Periostin may play an important role in invasion, angiogenesis and metastasis, as demonstrated by using cell lines and mice model.
Given the strong correlation of CD44 expression to the basal-like subgroup of breast cancers, a series of in vitro experiments was performed by using cell lines representative of this subtype, primarily by using the invasive MDA-MB-231 cell line and a highly metastatic derivative MDA-MB-231 cell line (MDA-MB-231Hi).
False positive results and off-target effects can be partially filtered by using cell-line transcriptome expression data, and we have presented a web-tool to enable Drosophila cell-based RNAi screeners to filter screen results.
An enormous amount of time, cost and effort can be wasted by scientists using cell lines that are either misidentified or contaminated.
The early enthusiasm for incretin-mediated preservation or enhancement of β-cell mass and function was based on extensive preclinical studies predominantly in rodents and complemented by studies using cell lines and islets (3, 4).
The LNCaP cells, in turn, had become contaminated by 22Rv1, another widely used cell line that is now known to harbor XMRV.
