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The proliferation of cBMSCs seeded on the pure CSH and Mg/CSH composite cements was evaluated by using cell cycle test based on flow cytometry.
This study investigated the in vitro anticancer effects of the ethanol extracts against human epithelial MCF-7 breast cancer cells in a dose-dependent manner (1 50 μg/ml) by using cell cycle analysis, viability assay, annexin V/PI staining, TUNEL method and expression determination of apoptotic and adhesion relevant proteins.
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Here we report a simple and robust system to enhance genome engineering by HDR in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.
The distribution of the cells was evaluated by using the cell cycle template with (FloJo).
DNA content was analyzed in FACS Calibur (Becton Dickinson, Franklin Lakes, NJ, USA), and data analysis was performed by using the cell cycle tool of FlowJo software (Tree Star, now part of FlowJo, LLC, Ashland, OR, USA).
Cell cycle was analyzed by FACScan using Cell Quest software (Becton Dickinson).
Cell cycle was analyzed by assessing the cell cycle phases using flow cytometry (FACScalibur, using Cell Quest software; Becton Dickinson) according to Nicoletti et al. [25].
Krebs cycle kinetics was evaluated by C-NMR isotopomer analysis using cell perchloric acid extracts.
(b) The cell cycle distribution was evaluated by flow cytometry using cells stained with Hoechst 33342.
Proliferation is also incorporated into histologic grading, either by counting mitotic figures (i.e., modified Nottingham Bloom Richardson score) or by developing a mitotic index using a cell cycle regulated biomarker such as Ki-67 [ 24– 26].
DNA content was analysed by FACSCALIBUR (Becton Dickinson, San Jose, CA, USA), using ModFitLT cell cycle analysis software (Verity Software House Inc., Topsham, ME, USA).
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