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Cell viability was measured by using Cell Counting Kit-8 (Dojindo Laboratories, Kumamoto, Japan).
We carried out this test by using Cell Counting Kit-8 (CCK-8) which is an upgrade alternative to MTT assay.
Cell viability was then determined by using Cell Counting Kit-8 (Sigma, Switzerland) according to the manufacturer's protocol.
In addition, the cell viability on AS and mineralized AS was determined by using Cell Counting Kit-8 (CCK-8, Dojindo).
Proliferation and cell viability analysis was performed by using Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technology) according manufacturer's instructions.
Cell proliferation was analyzed by using Cell Counting Assay Kit-8 (CCK-8) (Dojindo Molecular Technologies, Gaithersburg, MD) according to the manufacturer's protocol.
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Cell-proliferation assays were performed to analyze the proliferation potential of parental, empty vector and PRDX6-transfected cells by using Cell-Counting Kit (CCK -8 (Dojindo, Kumamoto, Japan).
Cell viability was determined by using cell counting-8 (WST-8) kit (Sigma Chemical Co).
Cell viability was determined by the WST assay using Cell Counting Kit-8 (Dojin Chemical Co., Kumamoto, Japan) according to the manufacturer's instructions.
Cytotoxicity induced by the nanomachine was measured using cell counting kit (CCK -8 assay.
The lack of cytotoxicity was confirmed by establishing viability of ADSCs using cell counting kit-8 (CCK-8) and live/dead assays.
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