Sentence examples for by using cDNA derived from inspiring English sources

For each gene, standard curves were generated by using cDNA derived from 100, 33.3, 11.1, 3.7 or 1.2 ng of total RNA.

Relative transcript abundance was analyzed by Q-PCR using cDNA derived from 2.5 ng of total RNA, gene-specific primers and ABsolute QPCR SYBR Green Mix (ABgene) with ROX as an internal reference.

Full-length coding sequence of NtGNL1a was amplified using cDNA derived from 3-days old BY-2 cell culture as a template.

Standard curves were generated for each gene, using cDNA derived from a serial 3 fold dilution of cDNA derived from one of the biological replicates.

Standard curve was obtained using cDNA derived from LPC and HPC for the MRF MyoD, myogenin and UCP-1.

Each QPCR was performed in triplicate using cDNA derived from three independent experiments.

The full-length human caspase-3 gene was amplified by PCR using cDNAs derived from human epidermoid carcinoma A431 cells as templates, and primers containing NdeI/ XhoI sites (NdeI, 5'-GGAATTCCATATGGAGAACACTGAAAACTCAG-3' XhoI, 5'-CCGCTCGAGGTGATAAAAATAGAGTTCTTTTGTG-3').

Bait fragments were obtained by RT-PCR using cDNAs derived from healthy lymphocytes, with the primers indicated in Additional file 1 and were subsequently cloned into the Gateway pDONR201 (Invitrogen) vector.

All reactions were carried out in triplicate using cDNAs derived from 3 different osteoclast/osteocyte cultures.

As a control we used cDNA derived from the serotype D wild-type strain JEC21.

Two micrograms of total RNA was reversely transcripted into cDNA by using cDNA Reverse Transcription Kit (Invitrogen).