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The phenolic content of each nanoemulsion was examined by using both colorimetric assay and chromatographic analyses.
We acquired the 6 cheapest products that did not require a prescription and tested for active ingredient by using both colorimetric methods and HPLC.
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Production of was shown to occur over a period of about 30 h, and by using another colorimetric assay, H2O2 was shown to accumulate.
The total flavonoid concentration was determined by using a colorimetric method with minor modification (Bao et al. 2005).
Total hardness (TH) as CaCO3, calcium (Ca2+), magnesium (Mg2+), bicarbonate (HCO3−) and chloride (Cl) were analyzed by volumetric methods and sulfates (SO42−) were estimated by using the colorimetric method.
The assay was carried out by using a colorimetric, commercial kit (Calbiochem-Novabiochem Corporation, USA) according to the manufacturer instructions.
We have also investigated this phenomenon by measuring the activity of caspases by using a colorimetric caspases kit that measures the activity of caspases-3 in the samples.
Measurement of NO activity by using a colorimetric NO assay kit showed that Shh overexpression was associated with a concomitant increase in NO production in ShhMSCs (Figure 3C).
The cholesterol concentration in lipid raft fractions and membrane preparations was enzymatically determined by using the colorimetric method from Boehringer Mannheim following manufacturer's instructions.
Then the cells were harvested and caspase 8 activity was measured by using a colorimetric ApoAlert Caspase-8 Assay Kit (colormetric) from Clonetech Laboratories, Inc. (Mountain View, CA) according to manufacturer's instructions.
The nitric oxide (NO) content was measured by using a colorimetric assay kit (BioVision, CA, USA).
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