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ERα status was determined at the protein level by using biochemical methods (Dextran-coated charcoal method until 1988 and enzyme immunoassay thereafter) and was confirmed at mRNA level by real-time RT-PCR.
These results, obtained by using biochemical methods, were also confirmed by using the FRET technique (bottom boxes).
Members of SIG are difficult to differentiate by using biochemical methods and require molecular techniques for accurate species-level identification (3 ); therefore, misidentification of these pathogens could occur.
ER status was determined at the protein level by using biochemical methods (Dextran-coated charcoal method until 1988 and enzyme immunoassay thereafter) and was confirmed at mRNA level by RT-PCR.
Estrogen receptor (ER) and progesterone receptor (PR) status was determined at the protein level by using biochemical methods (dextran-coated charcoal method or enzyme immunoassay) until 1999 and then by immunohistochemistry.
ER and PR status was determined at the protein level by using biochemical methods (dextran-coated charcoal method or enzymatic immunoassay) until 1999 and later by using immunohistochemistry. Cutoff for ER and PR positivity was set at 15 fm/mg (dextran-coated charcoal or enzyme immunoassay) and at 10% immunostained cells (immunohistochemistry).
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These peptides were tested using biochemical methods and models of angiogenesis.
The pure isolate was identified using biochemical methods [18], and 16S rRNA analysis.
Using biochemical methods, Srivastava's group showed that NOTCH1 controls calcium pumping.
We then characterized this interaction using biochemical methods.
By immunohistochemistry, we may also be detecting primarily fibrils as opposed to soluble oligomeric fibrils, the latter may be optimally detected using biochemical methods [ 35].
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