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We extended these studies by using biochemical cell fractionation.
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We could show here, by using biochemical and cell biological approaches that transiently expressed Arabidopsis U1 snRNP-specific proteins localise to the nucleus and efficiently assemble into mature snRNPs.
Here, we have used biochemical, cell biological and molecular genetic approaches to demonstrate that β8 integrin (Itgb8) and neuropilin 1 (Nrp1) cooperatively promote CNS angiogenesis by mediating adhesion and signaling events between neuroepithelial cells and vascular endothelial cells.
The docked poses were sorted and the top-scoring molecules were tested experimentally by using biochemical and cell-based assays as described above.
By using biochemical and genetic tools we have monitored the localization and degradation of endogenous and chimeric proteins in live primary cells by confocal microscopy and ultra-structural analysis.
Other important biochemical properties were performed by using biochemical test kits (KB013 HiMEDIA HiBacillusTM identification kit).
We analyzed the relative abundance of PACER within the cell using biochemical fractionation of U937 macrophages followed by qPCR.
Describes toxic effect mechanisms using biochemical processes influenced by xenobiotics as an example, and.
Cell apoptosis rate was determined from tumour sections subjected to in situ TUNEL assay (Gavrieli et al, 1992) by using a cell death detection kit (Roche Biochemicals, Mannheim, Germany).
The measurement of glucose and the other biochemical parameters was carried out by using a biochemical autoanalyzer (Cobas Integra 400).
Apoptosis in these cells was quantified by using a cell-death detection ELISA kit (Roche Molecular Biochemicals, Laval, Quebec, Canada) according to the manufacturer's instructions.
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