Sentence examples for by using as template from inspiring English sources

The other probe (P2) was generated by using as template, a 954 bp region of the T-DNA obtained by Nco I digestion (Figure 2d).

Positive and negative control experiments were performed by using as template either genomic DNA or DNase-treated RNA in the absence of reverse transcriptase, respectively.

The GFP gene was PCR amplified by using as template the GFP-C-λ phage, described in this study.

The anti-CEA scFv antibody-encoding DNA fragment was PCR amplified by using as template the α-CEA-N-λ phage [ 10].

Incomplete cDNAs were derived by PCR, using as template the cDNA generated by reverse transcription.

The mutant Rab11 cDNA was obtained by PCR using as template genomic DNA from flies containing UAS-Rab11N124I [45], and the primers Rab11F (5'-AAAGGCGCGCCATGGGTGCAAGAGAAGACGA-3') and Rab11R (5'-AAAGGCGCGCCTCACTGACAGCACTGTTTGC-3').

The methylation status of promoter region of ZMYM3 X-linked gene subjected to XCI (DXS6673E locus) was analyzed by PCR using as template the genomic DNA previously digested with the methylation sensitive enzymes HhaI and RsaI (New England Biolabs).

KCTD6BTB (residues 10 110 of KCTD6) was amplified by PCR, using as template the human KCTD6 cDNA (NM_153331) (thermo Scientific) and cloned into the pETM-11 expression vector (Novagen).

In this work, the ArtinM coding region was amplified by PCR, using as template the cDNA clone pLL29 described previously [ 16].

The coding region of the ArtinM lectin was amplified by PCR using as template the cDNA clone pLL29 previously described [ 16].

The CiHox1 genomic fragments 1UP2.4, 1intro3.6 and 1D1.7 were amplified by PCR using as template the cosmid clone MPMGc119H2170 isolated from a cosmid library prepared by the Reference Library Database [ 48].