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Sensor recovery posed a great problem at room temperature, and the fast and complete recovery was successfully achieved by using appropriate thermal treatment protocol.
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Enzyme-resistant products were reamplified by PCR using 1 μl of the final RSM product (amplification for 10 12 cycles using appropriate thermal profiles).
Reverse transcription was then performed in a thermal cycler using appropriate amounts of total RNA.
To achieve low thermal budgeting, charge-plasma concept is employed using appropriate metal work function electrodes.
Measurement of extravascular lung water (EVLW) by using the lithium-thermal (Li-thermal) and single-thermal indicator dilution methods was compared with the indocyanine green-thermal (ICG-thermal) method in humans.
The spring constant was calibrated by using the thermal fluctuations method.
The spring constant was obtained by using the thermal noise method.
DNA was amplified by using a thermal cycler (Touchgene Gradient, Techne Inc., Burlington, NJ, USA).
Quantitative real-time PCR was performed by using the Thermal Cycler Dice System (TaKaRa).
By using an appropriate wavelength of laser light, all classes of engineering material may be machined irrespective of their physical and thermal properties.
PCR was performed by using a DNA thermal cycler for 30 cycles.
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