Sentence examples for by using appropriate primer from inspiring English sources

Laboratory tests were carried out to check the suitability of the chosen products on all related surfaces by using appropriate primer materials.

For each pts gene, upstream and downstream regions (about 500 bp) flanking the gene were PCR amplified from B. melitensis 16M genomic DNA by using appropriate primers (Table S2).

DNAse was inactivated at 65°C for 10 min. mRNA purity and integrity were checked by RT-PCR using appropriate primer pairs of known genes and also negative controls as described in Verjovski-Almeida et al. [ 5].

Late RT products (top) and 2-LTR circles (bottom) in cell lysates were quantified by qPCR as described in 'Materials and methods' using appropriate primer sets.

Genotypes of mice were determined by PCR on genomic DNA derived from ear biopsies using appropriate primer pairs (Supplementary material).

The mRNA expression profiles obtained by reverse-transcriptase PCR (RT-PCR) (Fig. 5) revealed that the DNA microarray data could be validated using appropriate primer design.

STE2 disruption at the targeted locus and removal of URA3 were verified by PCR using appropriate primers.

YopH deletions were done by PCR using appropriate primers.

Briefly, two fragments of approximately 900 bp corresponding to the flanking regions (including the 5' and the 3'end parts, respectively) of the target genes were amplified by PCR using appropriate primers (Table 4).

The purified DNA fraction was then analyzed by PCR using appropriate primers.

cDNA was amplified by PCR using appropriate primers (Supplementary Table S1) and subjected to direct sequencing.