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Spray dried loaded microspheres based on chitosan or chitosan derivatives were obtained by using appropriate preparative parameters.
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The fractions with high peroxidase activity were eluted with 0.1 M methyl-α-mannopyranoside, concentrated and further purified by using preparative sodium dodecylsulfate-polyacrylamide gel electrophoresis (preparative SDS-PAGE).
The electrocatalyst layer microstructure was varied by using different preparative conditions (320 360°C sintering temperature and 20 60% PTFE content).
A new, specially designed column has been developed for fractionation of supercritical fluid extract of rosemary by using a preparative supercritical fluid chromatography system (Prep-SFC).
The crude product was then purified by using a preparative HPLC system using MS and UV directed fractionation.
For both cases, the major diastereoisomer could be isolated as a colorless solid by using a preparative TLC plate (hexane/isopropanol=15:1 as eluent).
Nowadays, robust and flexible materials characterized by high porosity and surface area became available by using preparative procedures typical of the so-called reticular synthesis.
The compounds, purified from the fungal culture filtrate by using preparative HPLC-DAD, showed the same spectroscopic data of abscisic acid and 1′,4′-diol of abscisic acid.
Evidence is presented which indicates that proton discharge is rate limiting on these carbides, and that their performance is limited by scarcity of active sites and hence open to improvement by appropriate preparative technique.
A more recent method bypasses the need for these preparative steps by using anion-exchange chromatography to separate large-scale RNA transcription products on an FPLC column.
Column chromatography 5 1 LP/EtOAc, followed by a preparative TLC by using LP/EtOAc=3:1 and 1 % NEt3.
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