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The spatial/temporal regulation of genes can be modified by using appropriate endogenous regulatory elements, such as fruit-specific promoters.
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Defend negative arguments by using appropriate supporting examples.
Levels of phosphorylated endogenous (endog). JNK1 and JNK1CA (MKK7-JNK1α1), and HA and PKM2 were visualized by WBs using appropriate antibodies.
YopH deletions were done by PCR using appropriate primers.
The method developed here may be extended to visualize many other endogenous proteins in living cells using appropriate aptamer beacons.
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