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A grafting yield higher than 85% was obtained by using an amount of isocyanate terminated oligo(oxypropylene) of 0.2 mmol per gram of gelatin.
Yeast-display selection was performed by using an amount of yeast cells at least ten-fold larger than (i) the initial estimated naïve library size (1 × 109 unique clones) or (ii) the number of cells isolated from the previous round of either magnetic bead screening or flow cytometry sorting.
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Such a control can be achieved by using an optimum amount of weak acid.
In contrast, in the pre-mix strategy we grew E. coli cultures by using an equal amount of 2-13C and 3-13C pyruvase as the carbon sources for protein expression.
We demonstrate that by reducing the reaction temperature and time, and by using an optimal amount of iodine, we can maximize the yield of transient DMDS-1 adducts for alkenone structure determination.
Silica fillers are ideal fillers to investigate as the interaction at the filler interface can be easily controlled by using an appropriate amount of a suitable silane coupling agent.
By using an appropriate amount of acid along with either anionic or nonionic surfactants, about 80% of exfoliated, purified SWCNTs can be retrieved and stabilized in aqueous solution; the separated nanoplatelets can also be recycled.
To standardize the degradation power of the inoculum, the inoculum should be adjusted to 2.5% dry weight (± 0.5%), by using an appropriate amount of the anaerobic sludge.
53 This was experimentally verified by using an increased amount of water, which also was needed for the hydrolysis step.
For a first indication of whether it would be possible to perform selective chemistry on either of the two phosphorus heteroatoms, PPAs 5 a– c were subjected to oxidative conditions by using an excess amount of sulfur in CH2Cl2.
MEPs pushed to tighten the financing rules so that the fund is not used for coal-fired projects, except for district heating in the two poorest member states, provided that they offset the emissions produced by using an equivalent amount of free allowances (granted under a temporary derogation via article 10c of the legislation) for investments not involving solid fossil fuels.
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