Sentence examples for by using a yeast from inspiring English sources

By using a yeast two-hybrid system, we demonstrate selective interaction of the extradenticle homeodomain protein with certain Ultrabithorax and abdominal-A proteins but not with an Antennapedia protein or a more distant homeodomain protein.

By using a yeast expression system, mouse PAPST1 and PAPST2 proteins were shown to have PAPS transport activity with an apparent Km value of 1.54 µM or 1.49 µM, respectively.

The transgenic mouse line termed 143-YAChPPT-ALacZ containing the human Preprotachykinin-A (PPT-A) gene co-expressing the β-galactosidase (LacZ) reporter gene was produced by using a yeast artificial chromosome construct [14].

In fact, by using a yeast two-hybrid system to identify integrin α1 on cell membrane as a PRL-3-interacting protein, Peng et al. [24] demonstrated that PRL-3 could down-regulate the tyrosine-phosphorylation level of integrin β1, hence activating the MAPK pathway.

The proteins interacted with GT-4 were identified by using a yeast two-hybrid assay system.

We therefore addressed whether the interaction between Ndc80 and Dam1 was dependent on the Ndc80 loop region by using a yeast two-hybrid assay.

Predictions were confirmed by using a yeast-one-hybrid approach to identify interacting TFs belonging to the predicted TF families.

Predictions were confirmed by using a yeast-one-hybrid approach to select interacting TFs belonging to the predicted TF families.

The concentrations of inoculums were determined by spectrophotometer using a yeast cell suspension in sterile 0.85% NaCl with 10% transmittance at 520 nm.

Some of these problems could be addressed by using expression plasmids including a yeast signal peptide downstream of the cloning site and/or by using a different yeast species for protein production.

By using a fission yeast inducible nmt1 promoter, we were able to demonstrate the specific effects of viral proteins on fission yeast.