Time spent in moderate-to-vigorous physical activity was calculated by using a cut off of 420 countsecondsconds [ 22].
In October 2002, Uganda implemented a 5-year (2002 2006) accelerated measles control strategy that began with a vigorous attempt to interrupt all chains of measles transmission by using a 5-day countrywide vaccination campaign.
To provide initial insight into physical activity patterns and predictors of moderate to vigorous physical activity (MVPA) in youth with polycystic ovary syndrome (PCOS) by using a multisensor activity monitor.
Most people tend to use a vigorous back-and-forth motion, which is incorrect.
It was unethical, and probably logistically impossible, to conduct controlled field trials of vaccination at various periods into the incubation period during the many years that smallpox was being eliminated by using vigorous surveillance and vaccination of immediate contacts.
Cola drinks had carbon dioxide content largely removed by vigorous shaking using a stirring plate and placing a magnetic bar in a container filled with the liquid prior to being offered to the animals at room temperature.
The pellet was resuspended in 25 ml PBS and vortexed hard, which was followed by vigorous stirring using a magnetic stirrer for 30 min at RT with intermediate vortexing.
Intact colonic crypts were separated from the underlying layers by vigorous shaking, collected using a 40-µm nylon cell strainer (BD Biosciences) and treated with dispase (1.2 mg/ml) for 10 minutes to give a single-cell suspension.
Cells were monodispersed by vigorous pipetting, counted using a hemocytometer and then plated at the desired cell density onto sterile black-walled 96 well (Greiner Bio-One, Bio-One, Monroeroplates for ICW, iNCsterile 6-well plates (Corning, Lowell, microplates or on 8-chamber slides (VWR Int., USA) for fluorescence mICWoscopy experinentsterile
The peptides were concentrated by vigorous shaking at room temperature, using a modification of the method developed by Shevchenko [ 7].
