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Pickett et al. identified genes that they proposed are unique to the various brands by using a twofold threshold and a Venn diagram approach.
5 Subsequently, the trimer was dibrominated in the α-positions by using a twofold excess of NBS and then 5- methoxy carbonyl)thiophene-2-boronic acid, pinacol ester (5- methoxypled, by using Suzuki carbonyl thiophene-2-boronicmericarbonyl thiophene-2-boronicligomer 6.
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By using a modified twofold dilution scheme we determined the quantitative culture in CFU/ml.
By using a minimal twofold change (FDR ≤0.1) as the cutoff, we found 37 heat-induced genes and 17 heat-repressed genes in WT (in both biological repeats) (Fig. 2a, b; Additional file 1).
The LAP1-VHH-BS1 complex was formed by mixing the individual components using a twofold molar excess of VHH-BS1 and incubating for 30 min on ice, followed by size exclusion chromatography on a Superdex S75 column (GE Healthcare) equilibrated in buffer (10 mM Tris/HCl pH 7.4, 150 mM NaCl).
Standard curve was prepared for U6 snRNA using a twofold dilution.
We further 'filtered' these genes by using a cut-off threshold set at twofold change of expression, because this threshold could reflect a physiologically important change in gene activity, and a twofold change exceeded the average CV for all pairwise comparisons.
To enhance the mTurquoise fluorophore we frequently used a twofold accumulation.
We then fine-tuned the model with the images we prepared for training by using twofold cross validation.
As FCNs, we then fine-tuned the model with the prepared images by using twofold cross validation.
Raw skim milk was microfiltered twofold using a 0.1-μm ceramic membrane at 50°C.
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