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Thenar tissue oxygen saturation was measured by using a tissue spectrometer on 21 patients, and a vaso-occlusive test was performed before and 1 hour after transfusion.
It's not for the squeamish, but a step further down the path towards revenge is the Russian model who ended an argument with a woman who let her dog foul the pavement by using a tissue to collect it before flinging it into her convertible.
Deletion can be controlled both in time and space by using a tissue specific promoter to drive Cre expression.
Briefly, single cell suspensions from livers were prepared by using a tissue homogenizer and passing the homogenate through sterile nylon mesh (70 µM).
The infection status of the plants was verified in all different phases of the study by growing the fungi out from surface sterilized leaves, and by using a tissue print immunoblot (TPIB) assay [35].
Samples were then processed overnight in a vacuum infiltration processor E300, (Sakura Finetek, Torrance, CA), paraffin embedded by using a Tissue Tek TEC Embedding Center (Sakura Finetek), sectioned at 3 µM by using a HM315 Microm Microtome, and transferred to positively charged barrier slides (BioGenex, San Ramon, CA).
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The aim of this approach is not to replace damaged tissue with an implant or device but rather to prompt the patient's own tissue to enact a regenerative response by using a tissue-engineered construct to assemble new functional and healthy tissue.
We previously reported that APE1 is involved in melphalan resistance in the MM cell line KM3 by using a tissue-specific inducible APE1 silencing vector [ 15].
*AFLP, amplified fragment length polymorphism; CLO, Centrum voor Landbouwkundig Onderzoek; CCUG, Culture Collection of the University of Göteborg; NA, not applicable or available DNA of H. pullorum isolates was extracted by using a commercial tissue kit (DNeasy Tissue Kit, Qiagen).
Therefore, we examined the normal expression pattern of PSG9 in different tissues by using a multiple tissue northern blot, semi- and quantitative RT-PCR.
Total genomic DNA for all species was extracted from tissues by using a DNeasy tissue kit (Qiagen) following the manufacturer's protocol.
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