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The hydrogel toxicity was further evaluated by using a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay to examine the viability of skin sections under the experimental conditions.
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Sections were stained by using a fluorescent terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay kit (Roche Diagnostics, Mannheim, Germany) with counterstaining for DAPI.
As demonstrated in this paper, the targeting of proteins towards peroxisomes by using a C-terminal SKL sequence followed by fusion of modified peroxisomes with the plasmamembrane results in secretion of intracellular proteins.
We evaluated the possible contribution of IRE1 RNase to EREG expression by using a C-terminal truncated IRE1α mutant whose production in cells led to RNase inhibition while maintaining IRE1α autophosphorylation capabilities.
By using a N-terminal TIMP-1 protein with threonine-2 mutated to glycine (T2G) which differs from the wild-type protein by a single-amino-acid substitution, and reduces the inhibition constant of TIMP-1 for MMP-1 and MMP-3 by a factor of over 1000 and an ∼20-fold effect on MMP-9.
By using a passive two-terminal memristor, the memristor oscillator can be fully implemented on-chip with some simple circuit elements.
Recombinant BLyS/APRIL heterotrimers were produced by using a proprietary N-terminal trimerization domain [ 21].
Van den Berk et al. (2007) investigated the binding preferences of the 5 PDZ domains in protein tyrosine phosphatase PTP-BL by using a random C-terminal peptide lambda phage display library [ 40].
Our first method promotes the folding of the gene insert by using an N-terminal protein such as calmodulin that is well folded and expressed.
By using an N-terminal GFP-tag on Def1 instead, we ensured that both forms of Def1 could be detected.
Of relevance, Ottenheijm et al. recently examined nebulin protein expression in individuals with a NEB mutation similar to our zebrafish (exon 55 in-frame deletion), and found by western blot analysis that total nebulin levels were reduced approximately eightfold and that expression detected by using an N-terminal antibody was even more severely reduced (Ottenheijm et al., 2009).
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