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Herein, a label-free, isothermal, and ultrasensitive electrochemical detection of target DNA was developed by using a tandem polymerization and cleavage-mediated cascade target recycling and DNAzyme releasing amplification strategy.
Doubling of the copy number by using a tandem repeat of an antisense fragment did not further enhance the antisense effect, possibly indicating that a saturation point was already reached.
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We performed microsatellite genotyping of all A. fumigatus isolates for which the MIC of itraconazole was ≥16 mg/L (8 ) by using a short tandem repeat A. fumigatus assay, and we compared the results with those reported for the Netherlands (20 isolates) and other European countries (24 isolates) (Technical Appendix Figure).
Protein identification by peptide fragmentation using a tandem mass spectrometer equipped with a PCI-1000 ProteinChip Interface Single MS and MS/MS spectra were acquired on a tandem mass spectrometer equipped with a Ciphergen PCI-1000 ProteInterfaceterface.
The candidate proteins were excised from gels and prepared for identification by peptide mass fingerprinting using a tandem mass spectrometer equipped with a ProteinChip® interface.
A rapid quantitation method was developed and validated to determine the concentrations of these three lGnRHs by using a UPLC coupled tandem MS in positive ESI multiple reaction monitoring (MRM) mode.
The effects of Tv6 on the autophagy flux was studied by two different methods: LC3II quantification and by using a RFP/GFP LC3 tandem probe that allows the microscopic visualization of autophagosomes and autolysosomes.
In the future it may be possible to routinely screen newborns for mucopolysaccharidoses disorders, including Morquio A, by using a liquid chromatography tandem mass spectrometry (LC MS/MS) approach to detect reduced GALNS enzyme activity in dried blood spot samples [Spacil et al., 2013].
Finally, the amino acid sequences were determined by using a liquid chromatography-tandem MS (LC/MS/MS) system (see Fig S1 of Supporting Information).
Testing for S. aureus DNA was undertaken by using a commercial multiplex-tandem PCR targeting the S. aureus nuclease gene nuc, and methicillin-resistance gene mecA (MRSA Easy-plex assay kit; AusDiagnostics, Sydney, New South Wales, Australia), as recommended by the manufacturer.
The plasma and effluent concentrations of ciprofloxacin (molecular weight (MW) 331.3), meropenem (MW 383.5), piperacillin (MW 517.6), and tazobactam (MW 300.3) were determined by using a validated liquid chromatography with tandem mass spectrometry method that has been described previously [ 9]; see online supplement (Additional file 1) for more details.
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