Other screen readouts are conceivable –for example, it should be possible to uncover E3s that target a substrate of interest by using a substrate-reporter fusion protein.
Quantification of substrate phosphorylation was assessed using sandwich ELISA by using a substrate-specific capture antibody and an anti-phosphotyrosine detection antibody.
This may be improved further by using a decaying substrate feeding strategy (Maclean et al. 2008).
Moreover, this inhibition can be overcome by using a specific substrate concentration at which V max remains unaffected [35].
The passive sampler yielded highly reproducible Rs values and its PAH uptake was shown to be enhanced by using a collection substrate modified with a gas adsorbing coating (Tenax®) which was exclusively addressed being uptake from the gaseous phase.
In this paper, the cost of a light emitting diode (LED) package is lowered by using a silicon substrate as the base attached to the chip, in contrast to the conventional chip-on-board (COB) package.
Due to limited atomic assembly kinetics during the deposition process (e.g., by using a low substrate temperature), defects (point-, line-, and area-defects), supersaturated, and metastable phases can easily be obtained.
Osteopontin (OPN) was detected by using a chromogenic substrate for the secondary antibody according to the kit manufacturer's instructions.
Bound antibodies were detected by using a chemiluminescent substrate (NEN Life Science Products, Boston, MA).
