Exact(1)
Enhanced chemifluorescence blots were scanned by using a STORM fluorescent detection system (Amersham-Pharmacia) as described previously (16 ).
Similar(58)
Reactions were quenched at various time intervals with 0.6 M EDTA, and the products visualized by denaturing PAGE followed by autoradiography using a STORM 840 PhosphorImager and quantitated using ImageQuant 5.0 software (GE Healthcare, U.K .. Steady-state fluorescence experiments were performed on an Aminco-Bowman Series 2 luminescence spectrometer.
Proteins were analyzed by reducing or non-reducing 12% SDS PAGE gels with Sypro staining (Invitrogen, Carlsbad, CA) according to the manufacturer's recommendations and analyzed by autoradiography using a Storm 840 PhosphoImager.
Signals detected on northern blots were quantified directly by phosphor-imaging using a Storm 820 phosphorimager (Molecular Dynamics) or by scanning autoradiographs on a Umax Astra 1220 U scanner.
Gels were transferred to Whatman paper, dried at 80°C for 1 h, and analyzed by PhosphoImager Screen analysis using a Storm Imager (Molecular Dynamics, Sunnyvale, CA).
Methylation indexes were determined by densitometry of autoradiographs using a Storm PhosphoImager (Molecular Dynamics, Sunnyvale, USA).
The data were analyzed by scanning the Phosphor Screen using a Storm 820 Scanner (Amersham Biosciences), and the volume of each band were determined, and background subtracted, by computer integration analysis using ImageQuant version 5.2 software (Amersham Biosciences).
The labeled protein was visualized by scanning the gel for fluorescence using a Storm 840 fluorescence scanner with 450 nm excitation wavelength and 520 nm long pass emission filter.
The western blots were scanned using a Storm 840 PhosphorImager scanner and quantified by densitometry using ImageQuant software (version 1.2; both from Molecular Dynamics , Inc CA, USA).
Positive hits were detected using a STORM Phosphorimager (Molecular Dynamics).
Hybridization signals were detected using a STORM Phosphorimager (GE Helthcare).
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