Sentence examples for by using a sterile from inspiring English sources

The spore suspension (1 ml) was applied by using a sterile sprayer.

After 2 days, three wells (5 mm) were prepared by using a sterile cork borer at equal distance around the mycelia growth of pathogenic fungi.

By using a sterile pipette, 0.6 mL of the broth culture of each test organism was added to 60 mL of molten agar, which was cooled at 45 °C, mixed well and poured into a sterile Petri plate.

The outer edge of each sample was removed to minimize the variability caused by the un-patterned sidewalls by using a sterile 8 mm biopsy punch (VWR International); the 8 mm samples were then aseptically loaded into a 15 mL conical tube containing 2 mL sterile Dey-Engley broth (Sigma Aldrich).

A 0.025% sterile collagenase solution (Boehringer, Mannheim, Germany) was injected through the apical opening into the dental pulp cavity by using a sterile 29G needle and incubated at 37°C for 30 minutes.

By using a sterile background soil, we minimized the presence of contaminant soil biota in our pots and were able to provide equal soil abiotic growing conditions for our study plants.

All individuals were sacrificed by decapitation using a sterile razor.

Thereafter, the mucosa was collected by scraping using a sterile glass microscope slide at 4°C [ 26, 38], rapidly frozen in liquid nitrogen, and stored at −80°C until analysis.

Cross-contaminations among rams were prevented by using a plastic sterile and disposable liner inside the artificial vagina, connected to collection tube.

After complete solidification of the agar on plates, wells were punched out of the agar, by using a clean sterile cork borer.

Trained study personnel collected the nasopharyngeal swabs from children and parents by using a flexible, sterile, dry cotton-wool swab transnasally, according to World Health Organization standard procedures (14 ).