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Serum samples were tested by using a starting dilution of 1 20.

Crystals of recombinant BfrB were grown at 293 K by using a starting protein concentration of 11 mg/ml from a solution of 20% PEG 3350 in 0.1 M Tris pH 8.5.

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Because hRev1 failed to extend the 17-mer primer past the lesion site within 20 h, we chose to evaluate the ability of hRev1 to bypass the 8-oxodG lesion by using a standing start primer extension assay.

Saliva was collected for 10 minutes from the oral cavity of individual mice by using a micropipette starting 1 minute after injection of the secretagogue.

The conclusion from these trial structures is that it is not possible to generate a dimeric model that is crystal-chemically consistent with known calcium silicate hydrates by using an orthotobermorite starting structure that has bridging tetrahedra that are adjacent to one another or that are staggered by b/2.

In this approach, the candidate parameter settings recommended by a metalearning algorithm are used a starting point for further optimization using Tabu Search or Particle Swarm Optimization techniques, with great success.

Place the tube(s) in a PCR machine when the temperature of the block is at about 75°C (manual hot start; this step can be avoided by using a hot start Taq polymerase).

Bias was minimised by using a similar start time and ensuring that all observers were familiar with the study protocol.

Our third method eliminates any dependence of the fluorescent protein reporter on the folding of the gene insert by using a stop and start sequence for protein translation.

PAUP* 4.0b10 was used to obtain the maximum likelihood trees by using a neighbor-joining starting tree followed by tree-bisection reconnection branch swapping (28).

By using, as a starting point, a number of the current tools and techniques which attempt to obtain 'the value' of information, it is proposed that an assessment or filter mechanism for information is needed to be developed.

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