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20 In the second scenario (scenario ii) we investigated the impact of our assumption that MRI targeting has no impact on men without clinically significant disease (by using a specificity of 80% for MRI-targeted biopsy as reported by Kasivisvanathan et al 19 rather than our base case estimate of 90%).

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To overcome this issue, we identified genes that are likely expressed exclusively in a single sex by using a sex-specific metric of tissue specificity: (1) where E i is mean expression of tissue i and Emax is the maximum of the tissue-specific mean expression across all tissues (Yanai et al. 2005).

As a final step, antibody specificity was confirmed by using a highly specific MERS-CoV microneutralization assay as described (6 ).

The subsequent PCR gets its specificity by using a unique MMTV-LTR-related oligonucleotide and a splinkerette-specific oligonucleotide, which is only able to bind to the DNA if extension of the MMTV oligonucleotide occurs.

The focus of this report is to describe the qualification and performance characteristics of an ELISA for anti-PA IgG antibodies and enhancement of its specificity by using a second-stage CI-ELISA.

To assess the goodness-of-fit of the final model, we plotted sensitivity and specificity by using a receiver operating characteristic curve and estimated the area under the curve (c-statistic).

We further validated our predictions with the natural PDZ domains by using a mutagenesis dataset from a domain specificity study [ 13] as described in the Materials and Methods section.

Specificity was determined by using a panel of 328 Bacillus strains; sensitivity was determined by probit analysis.

Specificity was controlled by using a mutant (hereafter termed 3R) in which three hydrophobic residues in the TMD were changed to arginine.

Sensitivity and specificity were calculated by using a range of IFA endpoint cutoff titers for positivity, and binomial 95% confidence intervals (CIs) were calculated.

Positive (PPV) and negative (NPV) predictive values for mortality and false-positive rates (FPR; 1-specificity) were calculated by using a binomial 95% CI.

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