We addressed this by using a site-specific definition of P/HTN for Bt20 and adjusted for age in all models.
While this dramatic change is to some degree the result of using absolute, rather than geometric means, this is primarily caused by using a site-specific estimate of light trap efficiency rather than an external estimate from northern Tanzania.
9 Plasmid encoding the NES-deficient mutant FLAG-CALMNES4A-AF10 and FLAG-CALMNES4A were generated by introducing four point mutations into the CALM NES sequence by inverse PCR using a site-specific mutagenesis kit (Toyobo, Osaka, Japan).
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An example of a classical genetic approach is the work by Valens et al [ 5] who have used a site-specific recombination system in order to reveal spatial proximities of distant DNA sites.
The 3′-UTR of Keap1 was amplified using the following primers: 5′-TCATACTAGTGGCACTTTTGTTTCTTGGGC-3′ and 5′-GCATTAAGCTTCAGGGTGAAAGACACTAG-3′ and cloned into pMiR-Report vector (Ambion) digested with HindIII and Spe I. We also generated three bases mutation in the predicated target site for miR-200a by using a QuickChange site-specific mutagenesis kit (Stratagene).
By using a very site-specific drug, performing for the first time simultaneous measurements of CMRglc, rCBF, resting state BOLD low frequency oscillations, and analyzing both dexmedetomidine-induced loss of consciousness and recovery, we have been able to identify a minimal disruption of neural networks that is shared between altered consciousness states.
However here we showed that by using a specific phosphorylation site (pThr187) antibody, p27 is detectable also in proliferative compartments of normal, dysplastic and neoplastic tissues.
By using a specific phosphorylation site antibody, p27 expression may be detected in the proliferative compartments of squamous and intestinal epithelia, of lymph node germinal centres, of trophoblastic villi, of cervical and colonic pre-invasive lesions and of several carcinomas types.
Truncated TcdA (amino acids 1 1874) was generated by using a specific endonuclease recognition site (bp 5620) in TcdA, which is located within the first repetitive sequence, and cloning of the resulting toxin fragment into the modified B. megaterium expression vector pWH1520 [14].
