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by Using a Serum, Says Dr. Vincent of France.
Inactivated bat serum specimens were screened for NiV antibodies by using a serum neutralization test.
Positive I-ELISA and HI samples were confirmed by using a serum neutralization (SN) assay (7, 8 ).
To demonstrate more detailed antigenic reactivity, Western immunoblotting of rickettsiae was conducted by using a serum specimen from day 20.
We suggest that further study by using a serum free medium is required to investigate how AS activates the PI3K/Akt/mTOR pathway.
cImmunofluorescent antibody test was performed by using a serum dilution of 1 50, and the intensity of staining in SARS-CoV infected cellSARS-CoV infectedk infecellscells, was indicated butthe signot, winh +++ representing the strongest signal observed.
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Serum antibody levels were calculated from standard curves derived by using a standard serum pool with an assigned value of 100 U; specimens below the curve were assigned the lowest possible value (1 U).
Tubulin was detected using a monoclonal antibody (Sigma, 1∶2000) and endogenous TPI was detected by using a polyclonal serum ([32], 1∶5000).
Effects of proliferation were controlled for by using a reduced serum medium (3%FBS) and monitored via cell count.
All 1,488 V. parahaemolyticusisolates were serotyped by slide agglutination by using a commercial serum (Denka-Seiken Ltd., Tokyo, Japan); 47 serotypes were detected.
-Is the test diagnostic by using a single serum sample or we should have paired sera to show a rising antibodies titer?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com