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Caspase activity was measured in triplicate by using a respective Caspase-Glo 3/7, −8 and −9 assay kits according to the manufacturer's instructions.
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Serum levels of IL-6 were also measured in mice subjected to bleomycin injections and passive or active immunization as well as in their respective controls by using a U-cytech sandwich mouse IL-6 ELISA kit (U-cytech Biosciences, Utrecht, The Netherlands).
The cut-off levels of serum GMI for predicting mortality at the remaining time points were determined by using an ROC curve; the respective areas under the curve at the initiation of antifungal therapy and 1 and 2 weeks after antifungal therapy were 0.723, 0.812, and 0.795, respectively.
We obtained these spectra of the nanorods after separating them from their respective substrates by using an ultrasonicator.
We obtained these images and spectra of the nanorods after separating them from their respective substrates by using an ultrasonicator.
After establishing the relative amounts of COX-2 protein expressed by the E1A stable transfectants, we investigated whether those transfectants were more sensitive to celecoxib than their respective controls by using an MTT assay.
A minimum to maximum range was also identified by using the respective values within a specific age group.
Various simulations are carried out by using the respective models under an environment designed by setting an obstacle in the behavior space.
With respect to worst-case assessments, relationships can be evaluated for 90 and 95%% quantiles of leaf deposition values in an analogous way by using the respective quantile values in the above equations instead of the mean leaf value, (bar{D}_{text{i}}).
Antibodies were affinity-purified from the respective antisera by using the respective recombinant proteins coupled to a HiTrap NHS-activated HP column (GE Healthcare).
All 3-way and 2-way interactions between variables were assessed against a 10% significance level by using the respective P values from adjusted (Wald) hypothesis tests in the table of regression coefficients.
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