Sentence examples for by using a reconstituted from inspiring English sources

Having shown the ability of rIciA to inhibit helix opening in-vitro, experiments were designed to assess the ability of rIciA to actually inhibit DNA replication by using a reconstituted replication system.

Further, we determined that the intact TGF-β signal pathway was fully restored in AsPC-1 and CFPAC-1 stable SMAD4 reconstituted cells by using a SBE4 luciferase reporter assay, and by detecting the levels of SMAD2 phosphorylation after TGF-β1 treatment in AsPC-1 cells after SMAD4 restoration.

To probe the mechanism and selectivity of diolide formation, we sought to reconstitute ring formation in vitro by using a non-natural substrate.

The change in mass of reconstituted ferritin in the SWCNT nanocomposite shows reversible variation and stability of the ferritin/SWCNT nanocomposite on redox reactions was confirmed by using a coreless apoferritin and a Fe2+ chelating agent.

This can be achieved by using an inducible reporter, choosing the appropriate promoter, and reconstituting mice lacking cells of interest by injecting gene-modified Pax5 deficient pro-B cells.

Then desired homogeneous N-glycans are attached to the acceptor by using an activated sugar oxazoline as the donor substrate and a specific glycosynthase (mutant of endoglycosidase) as the catalyst to reconstitute a homogeneous glycoform.

By using NPCs reconstituted from Xenopus laevis egg extracts, we show that Nup98 is essential for maintaining the permeability barrier.

The present study is the first direct demonstration of lipid translocation mediated by MsbA, and avoids the ambiguity of whole-cell or membrane systems, where multiple proteins are present, by using purified reconstituted MsbA.

Using a purified, reconstituted system, we show that dMCPH1-B is ubiquitinated by APCCdh1, indicating that the effect of APC on dMCPH1-B ubiquitination and degradation is direct.

Further confirmation of the null effect of TSA on nascent mRNA expression was obtained by using NT embryos reconstituted with fibroblasts carrying the OCT4 promoter-driven enhanced green fluorescent protein (EGFP) (Fig.  7a- c).

We studied the interaction of RSC with nucleosomes using a single nucleosome reconstituted on a 601 nucleosomal positioning element flanked by two relatively long DNA arms of 236 and 252 bp.