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We also examined the effects of ELH-treated or untreated CM from HT1080 cells on microvessel sprouting by using a rat aortic ring assay, in which endothelial cell proliferation, migration, and tube formation occur simultaneously.
The current study was designed to investigate potential causative mechanisms of this impairment by using a rat model in which presbycusis is inducible by administration of d-galactose (d-gal).
By using a rat model of single orotracheal instillation of whole gastric contents, we studied the time course of morphological and biochemical changes during injury and resolution, and evaluated whether repair involved long-term fibrosis.
The level of MHC class I was evaluated at the same time by using a rat anti-pan-MHC antibody, M1/42.
Fasting insulin levels were quantified by using a rat insulin ELISA kit (Mercodia, Uppsala, Sweden).
Detection of Shh was done by using a rat anti-Shh antibody (R&D Systems).
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High-sensitive C-reactive protein (hsCRP) was determined by using a rat-specific ELISA kit from Alpha Diagnostics International (San Antonio, USA).
BrdU incorporation was visualized by immunofluorescence using a rat anti-BrdU monoclonal antibody (1∶200, Abcam, Cambridge, UK) and an A488-conjugated donkey anti-rat antibody (1∶200, Invitrogen).
In a preliminary set of experiments, endothelial cells were first identified by immunofluorescence using a rat anti-mouse CD34 (Dako, clone MEC 14.7) and a mouse anti-human CD31 (Dako, clone JC70A); a goat FITC-conjugated anti-rat (Dako) and an Alexa Fluor® rabbit anti-mouse (Invitrogen, Paisley, UK) antibody were used as secondary reagents.
This inspired us to investigate the mutagenicity of comfrey in rat liver, a target tissue for its carcinogenesis, by using a transgenic rat mutational model (Dycaico et al, 1994).
In the spring we became infested with a rat population but were able to get rid of most of them by using a common rat repellent.
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