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To verify this hypothesis we depleted TBP-containing (TFIID) complexes from the immunoprurified TAF8-containing complexes by using an antibody raised against TBP.
The sst2A receptor was immunolocalized by using an antiserum raised in rabbit against the C-terminal segment 330 369 of the human protein (1/5000) that specifically recognized sst2A antigens in rat and human brain sections by immunohistochemistry as previously demonstrated [6], [53] [55].
By using an antiserum raised to UIF, we detected it in extracts from 293T cells as a 37 kDa protein.
In the present study, we performed affinity chromatographies on FCT and MCT by using an antiserum raised against a boPAG-2 preparation [ 33- 35].
Immunodetection was carried out by using a polyclonal antibody raised against maize TGZ protein expressed and purified from E. coli [18] as primary antibody diluted 1/5000, and a peroxidise-conjugated goat anti-rabbit IgG (AO545, Sigma-Aldrich, Spain) at 1/15000 dilution as the secondary antibody.
He had discovered Mr. Dominguez by using a technology that raises important questions about how people are recruited and hired, and whether great talent is being overlooked along the way.
Have a block heater, and save fuel by using a heater to raise the engine temperature instead of idling.
Gels were stained with Coomassie Brilliant Blue (Sigma Aldrich) or immunoblotted onto a nitrocellulose membrane (Amersham Biosciences) for detection of MagKatG2 by using a homemade polyclonal antibody raised against catalase peroxidase from the cyanobacterium Synechocystis PCC 6803.
In addition, the nasal turbinate samples were inoculated into Vero cell lines for 5 blind passages, and aMPV viral antigens were further detected by immunofluorescent assay by using a rabbit polyclonal antibody raised against a polypeptide located in the N protein of all aMPV subgroups (8 ).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com