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Briefly, DNA samples were diluted from 10 to 10 viral genomic copies (vgc)/μL by using a quantified RFAV DNA template and were amplified by using AV3 primers with the detection threshold of ≈100 vgc in a volume of 15 μL.
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Moreover, our assay could be easily adapted in a quantitative format by using a previously quantified RNA to create a standard curve to which results from unknown samples can be compared.
For quantification of relative expression levels, blots were scanned by using a densitometer (BioRad) and quantified with ImageJ software (NIH).
Each aliquot was thawed to measure 207 proteins that were quantified by using a Luminex bead-based multiplex immunodetection methodology.
RNA was quantified by using a molecular probe specifically designed for RNA (RiboGreen from Invitrogen).
The propagation phase is then quantified by using a conventional fracture mechanics approach.
Patient symptoms were quantified by using a disease-specific health-related quality of life questionnaire.
The latter were quantified by using a mathematical model for sphere contact quantification in randomly packed binary mixtures.
Partial interaction effects are quantified by using a proposed dimensionless parameter that depends mainly on the connection's stiffness.
The inter-category land transitions were identified and quantified by using a spatially explicit field-level resolution dataset.
The residuals of the initial regressions showed positive spatial autocorrelation that could be quantified by using a spatial probit model.
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