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Assays for the rate of killing effect was carried out for EtOAc fractions from roots and aerial parts against B. cereus ATCC 9634 by using a protocol of Olajuyigbe and Afolayan [22].
By using a protocol of repeated stimulus patterns and recording intracellularly from R1 R6s and LMCs (Fig. 1A), we observe that the amplitude distribution of voltages "flattens" and that frequency spectra "whiten" over time (Fig. 1B, C).
Parasagittal slices, 200 µm thick were cut in a vibratome (Vibroslice 752; Campden Instruments, Sileby, UK) and kept alive for at least 4 hours after preparation by using a protocol usually adopted for electrophysiological recordings that allows to analyse the cells several hours after cutting the cerebellum [38], [39].
Catalase (CAT) activity was measured by using a protocol adapted from [ 29].
DNA was extracted by using a protocol adapted from Lamour and Finley (27 ).
We simulated ring vaccination by using a protocol similar to that used in Guinea trial (3 ).
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The resulting plasmids, named as pHGBS and pHGBA, were introduced into B. subtilis 168 by electroporation using a protocol described previously (Siriphongphaew et al. 2012).
DNA was transfected into cells by electroporation using a protocol adapted from Maniatis.
The responses against RV1 were performed by GSK using a protocol derived from the one used for 89-12 (Table 4 ).
If necessary, inpatient labor induction was managed by using a standard protocol.
Patients then underwent motor-control analysis in which kinetic and polyelectromyographic data were collected by using a standard protocol.
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