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Protein labeling by using a protein tag and tag-specific fluorescent probes is increasingly becoming a useful technique for the real-time imaging of proteins in living cells.
We have overcome this limitation by using a protein scaffold provided by the avian pancreatic polypeptide (APP), a natural hormone of 36 amino acid residues.
After trying for 2 years, Brad St. Croix of the Hopkins group succeeded by using a protein called P1H12, which occurs mainly on endothelial cells, as a handle to separate out the cells.
One possible treatment is to create new routes for blood to get to the heart muscle by using a protein called fibroblast growth factor (FGF) to stimulate new blood vessel growth.
Equal amounts of protein as quantitated by using a protein assay kit (Thermo Scientific) were loaded onto a 18% SDS PAGE gel (29∶1 acrylamide/bisacrylamide). Protein samples were denatured at 95°C for 15 min prior to loading.
We compared antibody responses to monkeypox virus infection and human smallpox vaccination by using a protein microarray covering 92 95% (166 192 proteins) of representative proteomes from monkeypox viral clades of Central and West Africa, including 92% coverage (250 proteins) of the vaccinia virus proteome as a reference orthopox vaccine.
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To overcome the issue, we designed new local protein delivery system by using a protein-loaded, redox-active, injectable gel (RIG), which is formed by a polyion complex (PIC) comprising three components, viz., cationic polyamine-poly ethylene glycol)-polyamine-poly ethylenelymer possessinglycol -polyaminemoieties as side chains; anionic poly(acrylic acid); and a protein.
Next, we analyzed the connectivity of the genes correlating with cell size by using a protein-association network database.
Here, we identify this repressor which we call muscle growth regulator (MGR)—by using a DNA protein interaction screen based on quantitative mass spectrometry.
By using a chimeric protein fluorescent protein, we observed how Drp1 changes its localization from diffuse cytosolic to punctate mitochondrial localization.
Protein was quantified by using a BCA protein assay kit with bovine serum albumin as a standard (Pierce Chemical, Rockford, IL).
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