Sentence examples for by using a primary from inspiring English sources
The HMS silica samples were prepared in a water-rich neutral medium by using a primary amine as structure-directing agent.
To tell the sender the selected primary path d ap, the SCTP- Rev + receiver will inform the sender of d ap 's path identifier (pid), by using a Primary Path Switching Notification (PPSN) chunk, as shown in Fig. 3c.
The immunohistochemistry identification of human cells was performed by using a primary mouse anti-human nuclei monoclonal antibody (Chemicon), specific for all human cell types.
Expression of the target receptor on MA11 cells was confirmed by detecting receptor-binding of scFvMEL/rGel by using a primary rabbit antibody against gelonin and an Alexa488 labeled secondary goat anti rabbit antibody, while control experiment using incubation of the primary and the secondary antibody alone resulted in no fluorescing signals (Figure S5).
Identity of LC and its N-terminal fragments were confirmed by Western blots on nitrocellulose membranes that were prepared by using a primary polyclonal antibody against a 16-residue N-terminal sequence of BoNT/A LC and a peroxidase-coupled, goat anti-rabbit IgG (H+L) as the secondary antibody [13].
The fusion proteins were detected by using a primary α-poly-His mouse IgG (H1029, Sigma) which was then detected with a goat α-mouse IgG peroxidase secondary antibody (A2554, Sigma).
The protein levels of OPN in conditioned media were assayed by immunoblot using a primary antibody from Santa Cruz Biotechnology Santaa Cruz, CA).
Biotinylated samples were then detected by immunoblotting using a primary monoclonal anti-biotin antibody (Sigma-Aldrich).
MMP-1 was detected by immunoblotting using a primary antibody against active/latent MMP-1 (MAB901, R&DSystems, Wiesbaden, Germany) and goat-anti-mouse IgG HRP as a secondary antibody (Sigma-Aldrich).
Brain slices were also prepared from animals 24 h after injection of ANG1005, and localisation was conducted by immunohistochemistry using a primary antibody against Angiopep-2, which also recognises ANG1005.
To investigate the effect of the withdrawal of the incentive on practices' performance, we reconstructed the relevant indicators by using a large primary care database, the Clinical Practice Research Datalink CPRDD).
