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The terminated incubation mixtures, as well as the matrix-matched calibration standards and QC samples, were subsequently processed by using a previously reported nanoprecipitation procedure [27] based on phase-separation induced nanoaggregation for rapid and complete separation of biocompatible NPs with minor modifications.
The effect of sequences within the p16 mRNA on d2EGFP protein expression was studied by using a previously reported tTA-regulated construct [17] which expresses a chimeric mRNA comprising the EGFP coding region and the entire p16 CR and 3'UTR (EGFP-p16 mRNA).
PCR amplification was done by using a previously reported procedure [ 11].
The genotype of gene-knockout strains was confirmed by using a previously reported protocol [ 14].
The lignin amount was calculated by using a previously reported calibration plot [ 45].
Piperaquine plasma samples were quantified by using a previously reported high-throughput assay consisting of liquid chromatography linked with tandem mass spectrometry detection (17).
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To minimize the systematic variation, all plasma samples were randomly analyzed by GC/TOFMS using a previously reported method [22].
The levels of cholesterol and 24S-OHC were measured by GC MS using a previously reported method.
Pathological parameters including activity indices (AI), and chronicity indices (CI) were assessed by renal pathologists using a previously reported system involving semi-quantitative scoring of specific biopsy features, with mild modification [ 17].
This was done by using a procedure previously reported to probe the sensing volume of gold nanospheres by adsorbing sequential layers of oppositely charged polyelectrolytes to the nanoparticle surface.
[F]FDOPA synthesis was performed by use of a previously reported procedure [ 21].
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