Sentence examples for by using a polymerase from inspiring English sources
We have developed a novel method for cloning gene family members by using a polymerase chain reaction technique.
The genotype frequency of this polymorphism was determined by using a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay.
Methods: The genotype frequency of this polymorphism was determined by using a polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) assay.
Mice were housed at room temperature (21°C) on a 12 h light/dark cycle, and genotyped by using a polymerase chain reaction assay [25].
All recovered isolates were subtyped by using a polymerase chain reaction (PCR) assay (9 ).
The β1AR Arg389Gly polymorphism was determined by using a polymerase chain reaction-restriction fragment length polymorphism assay, a modified PCR-technique described previously by Maqbool [ 19].
The gene was designed with E. coli codon bias and assembled by using a recursive polymerase chain reaction method.
The purified DNA was amplified by using a fluorescent polymerase chain reaction (PCR).
Genotypes of HBV were determined by using a multiplex polymerase chain reaction (PCR) assay developed in our laboratory 23 and confirmed by DNA sequencing.
By using a Taq polymerase with proofreading capability (AccuPrime Taq), we were able to significantly reduce the amount of primer dimer generated during the two-step PCR.
The presence of mef and/or ermB was determined by using a duplex polymerase chain reaction (PCR) (7), and mefA was differentiated from mefE by PCR-restriction fragment length polymorphisms (8).
