Sentence examples for by using a polyclonal from inspiring English sources
Immunohistochemical analysis was performed by using a polyclonal mouse ascitic fluid at a dilution 1∶750.
Tubulin was detected using a monoclonal antibody (Sigma, 1∶2000) and endogenous TPI was detected by using a polyclonal serum ([32], 1∶5000).
GFP fusion proteins were detected using a monoclonal GFP antibody (Roche, 1∶2000) and FLAG-tagged fusion proteins were visualized by using a polyclonal antibody directed against the FLAG tag (Sigma, 1∶5000).
Next, MFN2 was detected by using a polyclonal rabbit-anti-MFN2 (N-terminal) serum (Sigma), a goat anti-rabbit IgG-HRP conjugate (DAKO) as secondary antibody and a chemiluminescence-based detection system (ECL plus, GE healthcare).
Immunodetection was carried out by using a polyclonal antibody raised against maize TGZ protein expressed and purified from E. coli [18] as primary antibody diluted 1/5000, and a peroxidise-conjugated goat anti-rabbit IgG (AO545, Sigma-Aldrich, Spain) at 1/15000 dilution as the secondary antibody.
A subset of ticks was dissected, and midguts were screened for spirochetes by fluorescent microscopy by using a polyclonal antiborrelial antibody (18 ).
We next addressed whether systemic treatment with mU1 affected plasminogen activation in skin wounds by analyzing the proteins in skin wound lysates by immunoblotting using a polyclonal antibody that recognizes both plasminogen and plasmin [29].
Fractions (1 mL each) were collected, washed and analyzed by immunoblot using a polyclonal rabbit anti-IscU antibody [71], [72].
We then analyzed the proteins precipitated on SERT-Ab by W/B using a polyclonal phosphovimentin- pS56 -Ab, which reacts only with the phosphovimentin- pS56 -Ab.
Clones were selected using growth medium containing 1 mg/ml G418 (Roche), and APOBEC3G expressing clones were identified by immunoblotting using a polyclonal antibody toward human APOBEC3G (J. Lingappa, University of Washington).
GFP expression was confirmed by immunohistochemistry using a polyclonal GFP antibody at a 1 1000 dilution.
