Sentence examples for by using a poly from inspiring English sources
Polyadenylated cyclin D1 mRNA was generated by using a poly(A) tailing kit (Ambion).
The modulation of the inflammatory response was performed by using a poly(ADP-ribose) polymerase-1 (PARP-1) inhibitor [3-aminobenzamide (3-AB), 90 mg/kg, i.p.].
For sequencing of the 3'-end of viral RNAs, a poly A tail was added to viral RNAs by using a Poly(A) Polymerase Tailing Kit (Epicenter biotechnology, Madison, WI).
Total RNA was employed for cDNA synthesis by using a poly-T primer with Fermentas MuLV Reverse Transcriptase.
Briefly, mature microRNAs were poly-adenylated and cDNA were synthesized by using a poly-T primer with a 3′ degenerate anchor and a 5′ universal tag.
As diagrammed in Figure 5, both the LTc and LTi transcripts are tricistronic and are polyadenylated by using a common poly A (pA) signal at nt 122094 in the virus genome.
In pioneering studies, Politz and Pederson labeled endogenous polyadenylated RNAs by in vivo hybridization using a poly(T -oligonucleotide and demonsT -oligonucleotidependent diffusionandmotion [refs 39,40].
Oh et al. [79] also reported 6.59 % of PCE by using a blend of (poly[ 9,9-bis((60-(N,N,N-trimethylammonium)hexyl)-2,7-fluorene)-alt-(9,9-bis(2-(2-(2-methoxyethoxy) ethoxy)ethyl)-9-fluorene))] dibromide (WPF-6-oxy-F) and graphene oxide as a hole transport layer in PTB7:PC71BM-based OSC.
Here we show that RCs from Rhodobacter sphaeroides can be immobilized on gold surfaces with the RC primary donor looking towards the substrate by using a genetically engineered poly-histidine tag (His7) at the C-terminal end of the M-subunit and a NiNTA terminated self-assembled monolayer (SAM).
The fusion proteins were detected by using a primary α-poly-His mouse IgG (H1029, Sigma) which was then detected with a goat α-mouse IgG peroxidase secondary antibody (A2554, Sigma).
The cDNAs thus analyzed were synthesized by reverse transcription using a poly-dT primer; therefore, most of the clones showed canonical mRNA features and had ORFs.
