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By using a pipette, transgenic worms are then transferred to our substrates for lensfree on-chip imaging.
The nostrils were gently tickled by using a pipette tip to instigate sneezing and the expulsion of nasal mucus and nasal wash, which were collected into a Petri-dish and supplemented with 1 ml of media.
Furthermore, since the modules easily transfer from one container to another by using a pipette, they provide a convenient platform, in combination with live-imaging confocal analysis, for monitoring module morphogenesis and cell re-organization over time.
Haemolymph from the 3 triatomine species was collected from fourth instars using the following procedure: nymphs had their femur cut and the leaking haemolymph was collected by using a pipette, transferred to a microcentrifuge tube and centrifuged at 10,000 g for 3 min at room temperature.
Transfer the PBMC into a new 50 ml pp-tube by using a pipette.
A scratch through the central axis of the plate was gently made by using a pipette tip.
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Transfection was performed by electroporation by using a pipette-type microporator (Microporator system; Digital Bio Technology, Suwon, Korea).
Following incubation, the neurospheres were triturated: first by using a Pasteur pipette, then by using a 1 mL pipette tip, and finally with a 23 G syringe needle, with 10 to 15 minutes for each step as previously described [ 26].
SV was estimated directly by using a calibrated pipette (vol. = 25 ml, Diffco Din, Witeg, Germany) to fill the shell with distilled deionized water.
By using a sterile pipette, 0.6 mL of the broth culture of each test organism was added to 60 mL of molten agar, which was cooled at 45 °C, mixed well and poured into a sterile Petri plate.
Then, the samples were centrifugated at 1,500 rpm for 15 min., and for each sample 20 mL of supernatant was transferred in a sterile polyethylene tube (Thermo Scientific Nunc, 30x115 mm, 50 ml) by using a calibrated pipette (vol. = 25 mL, Diffco Din, Witeg, Germany).
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