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The stiffening of the microenvironment was created by using a peptide linker with additional tyrosine residues, which were susceptible to tyrosinase-mediated crosslinking.
Castillo et al., by using a peptide nanotube-folic acid modified graphene electrode, improve detection of human cervical cancer cells overexpressing folate receptors [90 96].
At the current time, the most sensitive way to detect these antibodies is by using a peptide, derived from the PM/Scl-100 protein, as the antigen in an ELISA, instead of complete proteins.
The concentration of of the cis form is maximized by using a peptide stock dissolved in trifluoroethanol containing 470 mm LiCl, and its hydrolysis is limited by the rate of cis-trans isomerization.
However, considering that it is an 18-aa peptide, we decided to evaluate the relevance of the terminal amino acid composition by using a peptide including only the 10 first amino acids.
ChIP was performed with anti-Myb monoclonal 1.1 antibodies (Millipore, Billerica MA), control non-immune serum (10 µl), anti-FLAG (Sigma, St . Louis MO or with a rabbit anti-peptide antiserum prepared by using a peptide (HQGTILDNVKNLLEFAE) from the c-Myb transcriptional activation domain as antigen (Ab 1493).
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To identify platelet proteins that interact with the C terminus of SERT, we used a proteomic approach based on peptide affinity chromatography by using a synthetic peptide corresponding to the last 26 amino acids from the C-terminus (586 630) of SERT.
Polyclonal anti-ABCG1 antibody, 4497, was made by Genscript using a peptide (TKRLKGLRKDSSSM, amino acids 374 to 387 of ABCG1) as an antigen as described in ref (11).
After digestion, residual detergents were removed from the digests using a strong cation exchange macrotrap (Michrom TR1/25108/53, Bruker Corporation, Fremont, CA, USA) followed by desalting using a peptide macrotrap (Michrom TR1/25108/52, Bruker Corporation) according to manufacturer's instructions.
The deacetylase activities of SIRT2 were measured by using a substrate peptide with one end coupled to a fluorophore and the other end to a quencher.
Peptidase activity was measured by using a fluorescent peptide substrate, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin (Suc-LLVY-MCA), as described (Hirano et al., 2006).
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